Synthesis and bio-activity evaluation of tetraphenyl(phenylamino) methylene bisphosphonates as antioxidant agents and as potent inhibitors of osteoclasts in vitro

A new series of tetraphenyl bisphosphonates have been elegantly synthesized by one-pot method and were characterized by elemental analysis, FTIR, 1H, 13C, 31P NMR, mass spectra and evaluated for their in vitro antibone resorptive activity by inhibiting growth of osteoclasts. Two bisphosphonates 3g and 3f showed marked inhibition ratio (8 μM and 10 μM) and emerged as lead compounds. All compounds were tested for their antioxidant (DPPH scavenging, reducing power and inhibition of lipid peroxidation). They exhibited potent in vitro antioxidant activity dose-dependently.

Graphical abstractA new series of tetraphenyl bisphosphonates 3a-j were elegantly synthesized in a one-pot method and evaluated for their antioxidant/antibone resorptive activity and were found to inhibit osteoclastogenesis.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► One-pot effective synthesis of TPM-BPs are reported. ► First to evaluate their antibone resorptive and antioxidant activity. ► Explore the importance of TPM-BPs in thereupetic applications.