| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1397942 | European Journal of Medicinal Chemistry | 2009 | 8 Pages |
Chitosan, (1-4)-2-amino-2-deoxy-beta-d-glucan, is a deacetylated form of chitin, an abundant biodegradable, positively charged natural polysaccharide. Chitosan is used for antigen delivery through mucosal barrier due to its ability to disrupt tight junctions. Here we produced new water-soluble low-molecular weight chitosan (LMW-Chi) lipid derivatives and compared their ability to stimulate humoral response with the effect of unmodified LMW-Chi or its oligosaccharide derivatives. LMW-Chi effectively penetrated into macrophage-like, lymphoid and epithelial cells. It also stimulated in mice IgG production to model proteins delivered either by subcutaneous or intranasal routes. Adjuvant effect of chitosan derivatives was comparable to or lower than that of unmodified LMW-Chi. Thus, it is possible that adjuvant effect is induced by unmodified glucosamine units of chitosan.
Graphical abstractThe conjugates of low-molecular weight chitosans with fatty acids and carbohydrates were compared for their ability to stimulate IgG response in mice to model proteins delivered via subcutaneous or mucosal routes.Figure optionsDownload full-size imageDownload as PowerPoint slide
