| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1426125 | Journal of Controlled Release | 2009 | 5 Pages |
Abstract
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.
Graphical abstractPhoto-dependent gene silencing using several dyes, photopatterned RNAi, and single-cell RNAi was demonstrated.Figure optionsDownload full-size imageDownload as PowerPoint slide
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Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Tamaki Endoh, Masahiko Sisido, Takashi Ohtsuki,