Article ID Journal Published Year Pages File Type
1426125 Journal of Controlled Release 2009 5 Pages PDF
Abstract

In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with different excitation wavelengths. Additionally, we use light from a halogen lamp and a photomask to produce photopatterned RNAi, and laser light to trigger single-cell RNAi on cell culture plates.

Graphical abstractPhoto-dependent gene silencing using several dyes, photopatterned RNAi, and single-cell RNAi was demonstrated.Figure optionsDownload full-size imageDownload as PowerPoint slide

Related Topics
Physical Sciences and Engineering Materials Science Biomaterials
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