Article ID Journal Published Year Pages File Type
1426623 Journal of Controlled Release 2008 10 Pages PDF
Abstract

We report a method to load proteins into polymer-based sustained-release systems without exposing them to water–oil or water–air interfaces, factors known to denature proteins. By dispersing a dextan solution containing a protein into a PEG solution containing small amount of alginate, a stable aqueous–aqueous “emulsion” was formed. The poly-anionic alginate generated a diffuse double layer around each dextran droplet to prevent them from contacting with each other and fusing to a block phase. Proteins distributed in the stabilized dextran droplets by preferential partition favoring dextran. Freeze-drying this emulsion resulted in protein-loaded dextran particles, 1–2 µm in diameter and 1.6 g/cm3 in density. The particles were harvested by washing the lyophilized powder using organic solvents to remove the PEG continuous phase. An activity assay of encapsulated β-galactosidase indicated that protein activity was preserved during the particle-forming process including the step of sonicating the particles in organic solvents. The dextran particles also improved release profile and integrity of proteins when encapsulated in degradable polymer sustained-release systems. The aqueous–aqueous emulsion offers a convenient way to prepare solvent-resistant protein–polysaccharide particles that can easily be incorporated in a variety of polymer-based pharmaceutical dosage forms and medical devices such as microspheres, scaffolds and drug-eluting stents for sustained-release protein delivery.

Related Topics
Physical Sciences and Engineering Materials Science Biomaterials
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