| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1428237 | Materials Science and Engineering: C | 2015 | 7 Pages |
•Immobilization of Arthrobacter creatinolyticus urease on modified PAN membrane•Characterization of immobilized and free urease•Calibration of biosensor showed a wide detection range of urea from 1 to 100 mM.•Immobilized enzyme was stable for 70 days at 4 °C and reusable for 13 cycles.•Evaluation of biosensor for analysis of urea spiked milk sample
The extracellular urease from Arthrobacter creatinolyticus was partially purified by ammonium sulfate precipitation and immobilized on PAN [poly(acrylonitrile–methylmethacrylate–sodium vinylsulfonate)] membrane. The urease immobilized PAN membrane exhibited an activity of 97.92 U/cm2 under the optimum conditions of 1.0% enzyme concentration, 15% glutaraldehyde, 24 h immobilization time and temperature of 4 °C. The changes in surface morphology of the membrane after immobilization were studied by SEM and ATR-FTIR analysis. Immobilized membrane was associated with potentiometric electrode for calibration of biosensor and the results showed a linear response for wide range of urea concentration from 1 to 100 mM. The immobilized urease had good storage stability for a period of 70 days at 4 °C and could be effectively reused for 13 cycles. Urease immobilized membrane was also employed in analysis of urea spiked milk samples.
