Simple method for preparing glucose biosensor based on in-situ polypyrrole cross-linked chitosan/glucose oxidase/gold bionanocomposite film

•Chitosan–polypyrrole–gold bionanocomposite was synthesized for biosensor applications.•The biosensor seems to be fast to respond and sensitive.•The bionanocomposite used in this study is an effective platform to produce reliable biosensors.

A film of chitosan–polypyrrole–gold nanoparticles was fabricated by in-situ chemical synthesis method and its application in glucose biosensor was investigated. The obtained biosensor exhibited a high and reproducible sensitivity of 0.58 μA/mM, response time ~ 4 s, linear dynamic range from 1 to 20 mM, correlation coefficient of R2 = 0.9981, and limit of detection (LOD), based on S/N ratio (S/N = 3) of 0.068 mM. A value of 1.83 mM for the apparent Michaelis–Menten constant was obtained. The resulting bio-nanocomposite provided a suitable environment for the enzyme to retain its bioactivity at considerably extreme conditions, and the decorated gold nanoparticles in the bio-nanocomposite offer good affinity to enzyme.

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