Article ID Journal Published Year Pages File Type
1428660 Materials Science and Engineering: C 2014 5 Pages PDF
Abstract

•Chitosan is a choice material for scaffolds in regenerative medicine.•Schwann cells can produce the ECM components for nerve regeneration.•The laminin and collagen 4 are produced by Schwann cells.•RSCs grown on chitosan scaffolds secreted higher ECM than those grown on the plane.

Chitosan is a choice material for scaffolds in regenerative medicine. One of the applications is to bridge the damaged peripheral nerves. Previous studies showed that combination of chitosan conduits and cultured Schwann cells could increase the opportunity for re-connection of broken nerves. It has also been known that Schwann cells can produce the ECM components which are critical for nerve regeneration. In this study, we used the rat Schwann cells (RSCs) grown on porous chitosan scaffolds for quantitative analysis of ECM protein expression. The RSCs grown on chitosan scaffolds secreted higher amount of laminin and collagen 4 than those grown on the plane. The increased laminin and collagen 4 produced by Schwann cells could create a preferable condition for stimulating peripheral nerve regeneration.

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Physical Sciences and Engineering Materials Science Biomaterials
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