Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1428789 | Materials Science and Engineering: C | 2014 | 7 Pages |
•A new tertiary layered assembly named GN–CD–Cyt c was prepared.•Compared with GNs and GN–CD, GN–CD–Cyt c shows improved electron transfer rate.•GN–CD–Cyt c displays high supramolecular recognition capability.
This study aimed to develop a new graphene-based layered assembly, named graphene–cyclodextrin–cytochrome c with improved electron transfer rate. This assembly has combined high conductivity of graphene nanosheets (GNs), selectively binding properties and electronegativity of cyclodextrins (CDs), as well as electropositivity of cytochrome c (Cyt c). This assembly can also mimic the confined environments of the intermembrane space of mitochondria. A β-cyclodextrin (β-CD) functionalized GN (GN–CD) assembly was initially prepared by a simple wet-chemical strategy, i.e., in situ thermal reduction of graphene oxide with hydrazine hydrate in the presence of β-CD. Cyt c was then intercalated to the GN–CD assembly to form a layered self-assembled structure, GN–CD–Cyt c, through electrostatic interaction. Compared with GNs and GN–CD, GN–CD–Cyt c assembly displayed improved electron transfer rate and high supramolecular recognition capability toward six probe molecules.