| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1428861 | Materials Science and Engineering: C | 2013 | 7 Pages |
An electrochemical biosensor mediated by using 6-(Ferrocenyl) hexanethiol (FcSH) was fabricated by construction of gold nanoparticles (AuNPs) on the surface of polyamidoamine dendrimer (PAMAM) modified gold electrode. Glucose oxidase (GOx) was used as a model enzyme and was immobilized onto the gold surface forming a self assembled monolayer via FcSH and cysteamine. Cyclic voltammetry and amperometry were used for the characterization of electrochemical response towards glucose substrate. Following the optimization of medium pH, enzyme loading, AuNP and FcSH amount, the linear range for the glucose was studied and found as 1.0 to 5.0 mM with the detection limit (LOD) of 0.6 mM according to S/N = 3. Finally, the proposed Au/AuNP/(FcSH + Cyst)/PAMAM/GOx biosensor was successfully applied for the glucose analysis in beverages, and the results were compared with those obtained by HPLC.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Immobilized mediator in SAM layer and dendrimeric structure to expand surface area. ► Au nanoparticles for enhanced electron transfer. ► Satisfactory Limit of Detection with 0.6 mM.
