Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1429073 | Materials Science and Engineering: C | 2013 | 6 Pages |
The aim of this study was to show the feasibility and the performances of nanoparticle biosensing. A glucose conductometric biosensor was developed using two types of nanoparticles (gold and magnetic), glucose oxidase (GOD) being adsorbed on PAH (poly(allylamine hydrochloride)) modified nanoparticles, deposited on a planar interdigitated electrode (IDEs). The best sensitivities for glucose detection were obtained with magnetic nanoparticles (70 μM/mM and 3 μM of detection limit) compared to 45 μM/mM and 9 μM with gold nanoparticles and 30 μM/mM and 50 μM with GOD directly cross-linked on IDEs. When stored in phosphate buffer (20 mM, pH 7.3) at 4 °C, the biosensor showed good stability for more than 12 days.
► Glucose conductometric biosensor was developed using two types of nanoparticles. ► Glucose oxidase (GOD) is being immobilized, using layer-by-layer technique. ► MNP-based glucose sensors present a good stability.