Biosensor based on imaging ellipsometry for serotype-specific detection of Riemerella anatipestifer

In this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 × 103 CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1, 2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost.

► Biosensor based on imaging ellipsometry is utilized to detect Riemerella anatipestifer. ► The strategy of direct detection without labeling is adopted. ► The proposed method can detect R. anatipestifer as low as 5.2 × 103 CFU/mL. ► It also realized specific discrimination 1, 2, 4 and 14 serotypes in single experiment. ► But, the detection time is only 20 min required.