| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1430797 | Materials Science and Engineering: C | 2006 | 5 Pages |
Abstract
We use impedance spectroscopy technique to develop a rapid and sensitive antigen biosensor. The biosensor is based on the immobilization of antibodies onto an 11-Mercaptoundecanoic acid self-assembled monolayer. The high insulating properties of the thiol can be measured with cyclic voltammetry and impedance spectroscopy with a redox couple. The, the modified gold electrode is activated with 1-ethyl-3 (3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) ester for antibody immobilisation. The affinity interaction of the antigen–antibody can be measured with quartz crystal microbalance technique. Dissociation and association constant are determined by applying a Fortran program fitting for the experimental data.
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Authors
A. Tlili, A. Abdelghani, S. Ameur, N. Jaffrezic-Renault,