Article ID Journal Published Year Pages File Type
1440296 Synthetic Metals 2016 8 Pages PDF
Abstract

•An amperometric H2O2 biosensor based on poly(aniline-co-N-methylthionine) was created.•Poly(aniline-co-N-methylthionine) can enhance and fasten direct electron transfer kinetics of horseradish peroxidase.•Fast and sensitive detection of H2O2 with a wide linear range and long storage stability.

In this present work, we developed a novel hydrogen peroxide (H2O2) biosensor fabricated using electrochemical doping to immobilize hydrogen peroxidase (HRP) in a new conducting polymer, poly(aniline-co-N-methylthionine) (PAN-PNMThH). Amperometric detection of H2O2 was evaluated by holding the PAN-PNMThH HRP electrode at −0.25 V (versus saturated calomel electrode (SCE)). PAN-PNMThH showed excellent redox activity and high porosity and acted as an electron transfer mediator. The biosensor had a wide linear response range from 5.0 μM to 60.0 mM H2O2 with a sensitivity of 35 mA M−1 cm−2, a detection limit of 3.2 μM (signal-to-noise ratio of 3) and an apparent Michaelis constant (KM) of 2.79 mM. The biosensor possessed good analytical performance and storage stability.

Related Topics
Physical Sciences and Engineering Materials Science Biomaterials
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