| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1441986 | Synthetic Metals | 2011 | 5 Pages |
Abstract
In the present study, we demonstrate the first use of polypyrrole colloidal nanospheres (PPyCNPs) as an effective sensing platform for fluorescence-enhanced DNA detection by the following two steps: (1) PPyCNP adsorbs and quenches a dye-labeled single-stranded DNA (ssDNA) probe; (2) The subsequent hybridization of the probe with its target produces a double-stranded DNA (dsDNA) which detaches from PPyCNP, leading to recovery of dye fluorescence. The present system shows a detection limit as low as 1 nM and has a high selectivity down to single-base mismatch.
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Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Wenbo Lu, Yonglan Luo, Guohui Chang, Xiaoyun Qin, Fang Liao, Xuping Sun,