Crystalline size, microstructure and biocompatibility of hydroxyapatite nanopowders by hydrolysis of calcium hydrogen phosphate dehydrate (DCPD)

The crystalline size, microstructure and biocompatibility of hydroxyapatite (HA) bioceramic nanopowders synthesized by the hydrolysis of calcium hydrogen phosphate dehydrate (DCPD) at 75 °C for 1 h have been investigated. The crystallite size of HA nanopowders decreased from 23.1 to 13.7 nm when the concentrations of additional alcohol increased from 0 to 70%, whereas an increase from 13.7 to 14.9 nm was seen when the concentrations of additional alcohol increased from 70 to 90%. The morphology of HA nanopowders synthesized in a 2.5 M NaOH solution with 1.9×10−3 M CTAB and 70% alcohol was an almost spherical shape with a diameter of about 10.00 nm. The variation trends of cell viability inversely corresponded to the crystallite size and morphology of HA nanopowders.