In vivo optical tomography: From diffusion to ballistic

There are several novel applications in biology for optical tomographic approaches. The different specimens that can be imaged range from small non-scattering samples in the order of the millimeter range, to large scattering samples in the order of several centimeters such as mice. Depending on the level of scattering (ballistic, i.e. non-scattering or diffusive, i.e. highly scattering), there are different techniques used both to obtain the tomographic data and to render the 3D images. In this work we shall present two approaches used in our lab for in vivo imaging: optical projection tomography (OPT) for small and low scattering samples, and fluorescence molecular tomography (FMT) for large and highly-scattering samples.