Effects of Hydroxyapatite Nanoparticles on Devitrification of PEG-600 Cryoprotectant

To study the effects of hydroxyapatite(HA)nanoparticles on devitrification of cryoprotectant solutions, the crystallization of PEG-600(50%)solutions with HA nanoparticles of different particle sizes (20 nm, 40 nm, 60 nm) and concentrations (0.1%, 0.2%, 0.4%, 0.8%) were observed using a cryomicroscopy. Experimental results showed that devitrification onset temperature of PEG-600 solution containing 0.4% HA with the particle size of 60 nm increased by 10°C, the growth rate of ice crystal in PEG-600 solution containing 0.4% HA with the particle size of 20 nm and 0.8% HA with the particle size of 40 nm decreased by 35% and increased by 50% respectively when compared with the PEG-600 solution doped without nanoparticles. The structure of ice crystal in PEG-600 solution with HA changed remarkably.