Mutation Induction by DBD Plasma in Phosphate-solubilizing Bacteria Enterobacter Agglomerans

An efficient agar plate mutagenesis and screening technique for improving mutation frequency was established by using dielectric barrier discharge (DBD) plasma. Enterobacter agglomerans is well known as a phosphate-solubilizing plant-associated bacterium. In this study, DBD plasma was conducted toE. agglomerans mutation for improving the phosphate-soubilizing activity. The results showed that the phosphate-soubilizing activity of mutants increased compared with original strain, and the phosphate-soubilizing activity of the best mutants is 1.49-fold of original strain. It demonstrated that DBD plasma treatment has a high-efficient quality, and it will be a useful method of mutation.