Enhancing Biogas Production from Padauk Angsana Leave and Wastewater Feedstock through Alkaline and Enzyme Pretreatment

This work aimed to investigate the effect of pretreatment conditions on biogas production of Padauk Angsana leave (PA) or Pterocarpus indicus Wild with industrial wastewater at ambient condition. The biomass with %TS of 2, 5 and 10 were used in the study. 2%wt NaOH and cellulase were used as pretreatment agents. The one step and two steps pretreatments using NaOH, cellulase and both were compared. The amount of produced biogas was collected every 6 hrs in the period of 54 days. The results for untreated biomass case showed the 10%TS of PA achieved the highest biogas production. Similarly, for pretreated biomass using 2wt% NaOH, 10%TS of PA showed the highest biogas production. the best condition for methane production was found to be the two steps pretreatment, where 5%TS of biomass was soaked in 2%wt NaOH for 24 hours, followed by soaking in cellulase for 24 hours.