Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1541117 | Optics Communications | 2007 | 8 Pages |
Abstract
The solid immersion lens (SIL) as a tool for increasing the field confinement as well as providing optimal performance by aberration compensation in a confocal fluorescence correlation spectroscopy (FCS) system is illustrated here. Using Zernike polynomials we show that aberration compensation and the resultant pre-shaping of the incident wavefront enables near diffraction-limited performance. This is explained based on vectorial computations for high apertures in the Debye approximation. The obtained axial resolution parameters are compared with the obtained diffusion times in a SIL-FCS experiment for measurements in solutions done at the single molecule level.
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Materials Science
Electronic, Optical and Magnetic Materials
Authors
Ramachandra Rao, Jelena Mitic, Alexandre Serov, Rainer A. Leitgeb, Theo Lasser,