OsFKBP1 interacts with phytochrome B in rice

Part of the C-terminal of rice phytochrome B was used as bait to screen a cDNA library constructed from young rice panicles in a yeast two-hybrid screening. A positive clone was selected, and the sequence was characterized as encoding a FKBP protein, which is a putative developmental regulator. An in vitro pull-down assay using recombinant proteins and analysis of bimolecular fluorescence complementation in onion epidermal cells indicated that two proteins interact in vitro and in vivo. The results of reverse transcription polymerase chain reaction showed that two genes were co-expressed in the examined rice organs, suggesting that OsFKBP1 may play a role in light signal transduction in rice.