One-factor-at-a-time (OFAT) optimization of xylanase production from Trichoderma viride-IR05 in solid-state fermentation

The present study dealt with the production of enzyme xylanase by solid substrate fermentation using Trichoderma viride-IR05. Different substrates such as wheat bran, rice polish, rice husk, soybean meal, sunflower meal, sugarcane bagasse or corn cobs were evaluated for enzyme production. Of all the substrates evaluated, sugarcane bagasse was found to be best for enzyme synthesis. The substrate, sugarcane bagasse pretreated biologically, 2% H2SO4, 2.5% KOH or 3%H2O2. However 2.5% KOH gave maximum yield of enzyme as evidenced by the SEM analysis of the pretreated substrate. The cultural conditions were optimized for the production of xylanase in 250 ml Erlenmeyer flask such as incubation period (seven days), substrate concentration (10 g), liquid to solid ratio (11:10), initial pH of diluent (4.5), incubation temperature (30 °C) with inoculum size of 10%. Further supplementation of xylose, NaNO3 or tryptone and tween-80 as additional carbon source, nitrogen and surfactant improved (72.4 ± 1.42 U/g) the titer of xylanase by T. viride-IR05, respectively.