Imaging of articular cartilage - Data matching using X-ray tomography, SEM, FIB slicing and conventional histology

► Data from synchrotron X-ray tomography were compared to data from lightmicroscopy and to data from SEM/FIB imaging. ► Synchrotron tomographic measurements were performed at five different sample to detector distances allowing for phase contrast enhancements. ► A protocol was established allowing to identify a single cell in the tomographic tissue representation and preparing the same cellular structure for histology and image the cell in the same spatial orientation.