Article ID Journal Published Year Pages File Type
1589825 Micron 2008 9 Pages PDF
Abstract

We present a theoretical analysis of the image formation in conventional brightfield reflection microscopes with optical sectioning property via structured illumination. We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. Some effects of spherical aberrations are included in the analysis. The theoretical considerations are compared with and confirmed by experimental results.

Related Topics
Physical Sciences and Engineering Materials Science Materials Science (General)
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