Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1589990 | Micron | 2009 | 7 Pages |
Abstract
Remarkable results obtained while searching for suitable contrasting agents for tomographical imaging are presented. Book lungs, the respiratory organs of scorpions (Scorpiones, Arachnida), served as challenging objects in terms of fragility and preparatory requirements, as well in terms of size differences between the entire organ and its functional subunits (the lamellae). The problem of tissue contrast was investigated using various metals, but most successfully addressed using osmium. Gaseous osmium tetroxide exposure with subsequent ethanol treatment yielded the best results. A constant and thorough staining of the book lungs was achieved, easing book-lung visualization using 3D-reconstructions from μCT scans.
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Physical Sciences and Engineering
Materials Science
Materials Science (General)
Authors
Carsten Kamenz, Gerd Weidemann,