Fluorescence labeling of DNA based on photochemical ligation

Fluorescent labeling of oligonucleotides has been attracting interest in connection with the development of methods for distinguishing and detecting nucleic acids sequences. And photochemical ligation has the merit of the avoiding the need for additional reagents. Fujimoto et al. reported template directed DNA photoligation using 5-carboxyvinyl-deoxyuridine (CVU). Here, we describe the synthesis and photocrosslinking ability of fluorescent reporter analogue (Cy5) tethered CVU-containing ODN.