Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1635708 | Transactions of Nonferrous Metals Society of China | 2015 | 12 Pages |
Abstract
Porous titanium with porosity of 60% was prepared by metal injection molding (MIM), and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method. The effects of porous titanium coated with insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) gelatin microspheres on the function of MG63 cells were evaluated in vitro. The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity. The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg, respectively. The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg, respectively. The joint application of IGF-1 and TGF-β1 group, which promote adhesion, proliferation and differentiation of MG63 cells, is superior to a single application group.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Metals and Alloys
Authors
Liang-jian CHEN, Chang CHEN, Xue-yan QIAO, Kun YU, Li-zi XIE, Jun CAO, Bei-lei LIU, Yang YAN,