Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1636365 | Transactions of Nonferrous Metals Society of China | 2013 | 8 Pages |
Abstract
Leptospirillum ferriphilum YXW was isolated through serial dilution from mixed microorganisms enriched in AMD from Dexing copper mine in Jiangxi Province, China. It was mutated by ultrasonic, UV and microwave to collect more efficient strain for bioleaching of gold ore. Physiological and biochemical characteristics indicate that strain YXW is a strict chemoautotrophic microorganism, and the optimal condition for its growth is temperature of 40 °C and pH 1.5. After mutation by ultrasonic, UV and microwave, the density of bacterial cells reached 9Ã109, 8.4Ã109 and 4.3Ã108 mLâ1, increased by 291%, 265% and 87%, respectively, compared with the original culture. The bacterial total protein activity was improved by microwave and UV mutations, but was reduced by ultrasonic. Mutations had effects on bioleaching of gold ore in sequence of microwave > UV > ultrasonic. During gold ore bioleaching, the bacterial mutant after mutation by microwave had the best effect on the extraction rates of arsenic and iron, which were 19.6% and 17.7% higher than that of the original strain after bioleaching for 10 d, respectively. The results suggested that the effects of mutation on bioleaching of gold ore may not be mainly due to increase of bacterial cells density, but may be mainly attributed to the improvement of bacterial total protein activity.
Related Topics
Physical Sciences and Engineering
Materials Science
Metals and Alloys
Authors
Xue-wu YUAN, Xue-hui XIE, Feng-xia FAN, Weng-xiang ZHU, Na LIU, Jian-she LIU,