Electrospun nanoyarn scaffold and its application in tissue engineering

Silk fibroin (SF)/Poly(L-lactide-co-caprolactone) P(LLA-CL) nanoyarn scaffolds were prepared by dynamic liquid electrospinning. The scaffold morphology was observed by scanning electron microscopy (SEM) and mechanical properties of the scaffold were examined. L929 mouse fibroblasts were cultured on the nanoyarn scaffolds. Cell morphology, infiltration and proliferation on the scaffolds were investigated by SEM, hematoxylin-eosin (H&E) staining and methylthiazol tetrazolium (MTT) assay, respectively. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds. It was also observed that the nanoyarn scaffold significantly facilitated cell proliferation. Therefore, this work provides a promising approach to fabricate scaffolds for tissue engineering applications.

► We prepared a novel nanoyarn scaffold using electrospinning. ► The scaffold has excellent surface properties and porous structures. ► L929 cells show an oriented growth pattern and improved cell infiltration on the nanoyarn scaffold.