| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1649571 | Materials Letters | 2009 | 4 Pages |
Abstract
We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Anthony J. Di Pasqua, Richard E. Mishler II, Yan-Li Ship, James C. Dabrowiak, Tewodros Asefa,