Article ID Journal Published Year Pages File Type
1672177 Thin Solid Films 2009 7 Pages PDF
Abstract

Metal oxide [cerium oxide (NanoCeO2)]–chitosan (CH) nanocomposite film has been fabricated onto indium-tin-oxide (ITO) coated glass plate to immobilize cholesterol oxidase (ChOx) via physiosorption for cholesterol detection. Electrochemical studies reveal that the presence of NanoCeO2 in CH–CeO2 nanocomposite results in increased electroactive surface area for ChOx loading resulting in enhanced electron transport between ChOx and electrode. The ChOx/CH–NanoCeO2/ITO bioelectrode exhibits interesting characteristics such as detection range of 10–400 mg/dL, detection limit of 5 mg/dL, response time of 10 s, low Km value of 3.5 mg/dL and value of regression coefficient of 0.994.

Related Topics
Physical Sciences and Engineering Materials Science Nanotechnology
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