The self-assembled monolayer of saccharide via click chemistry: Formation and protein recognition

We prepared the saccharide-immobilized substrate via click chemistry. The azide-terminated saccharides were reacted by a facile metathesis reaction. The density of saccharides was controlled by the incubation time of SAM preparation. The saccharide–protein interaction was analyzed using the saccharide substrate. The interaction of the saccharide substrate with protein was strong and specific due to the glyco-cluster effects. The interaction with amyloid β was analyzed by the monosaccharide-immobilized substrate, and the sulfonated saccharides showed strong interaction.