| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1678327 | Ultramicroscopy | 2010 | 8 Pages |
Abstract
Combining scanning force and fluorescent microscopy allows simultaneous identification of labeled biomolecules and analysis of their nanometer level architectural arrangement. Fluorescent polystyrene nano-spheres were used as reliable objects for alignment of optical and topographic images. This allowed the precise localization of different fluorescence particles within complex molecular assemblies whose structure was mapped in nanometer detail topography. Our experiments reveal the versatility of this method for analysis of proteins and protein–DNA complexes.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Humberto Sanchez, Roland Kanaar, Claire Wyman,