Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1678698 | Ultramicroscopy | 2007 | 7 Pages |
Abstract
The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Mime Kobayashi, Koji Sumitomo, Keiichi Torimitsu,