Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1678730 | Ultramicroscopy | 2006 | 7 Pages |
Abstract
Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Matthias Wolf, David J. DeRosier, Nikolaus Grigorieff,