Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1683453 | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms | 2007 | 4 Pages |
The effect of gamma irradiation on the N-deacetylation of chitin to form chitosan was studied. Chitin from crab shells was irradiated up to 20 kGy and N-deacetylated in aqueous NaOH solution (40% and 60% w/w) at 60 and 100 °C for 60 min. The degree of N-deacetylation (DD) of non-irradiated and irradiated samples was determined by IR-band ratio method. It was found that higher extent of N-deacetylation was achieved for the chitin samples irradiated up to 20 kGy doses as compared to non-irradiated chitin. The DD values of chitin, prepared from non-irradiated and 20 kGy irradiated chitins by N-deacetylation at 60 °C with 40% NaOH for 60 min, were found to be 38% and 60%, respectively. The increase in DD by irradiation was interpreted as a result of reduction in molecular weight of chitin. Low dose irradiation of chitin has provided the possibility of its N-deacetylation into chitosan at much milder reaction conditions.