| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1684479 | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms | 2009 | 4 Pages |
Abstract
A 3 MeV He2+ beam was used to irradiate C8 (a flocculant-producing bacteria) with a fluence ranging from 1011 to 1013 ions/cm2. The effects on the survival ratio, TTC-dehydrogenase activity, flocculating activity and RAPD analysis are reported. The survival ratio curve caused by irradiation is proved to be “saddle-shaped”. Eleven mutants were obtained, all of which had a significant change in dehydrogenase activity and most showed a positive change in flocculating activity. RAPD measurements were used to analyse the DNA of mutants with a flocculating activity over 80%, which indicated that all their DNA had been changed by irradiation.
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Authors
L.L. Xu, Y.N. Yang, J.M. Xue, X.L. Ding, R.Q. Guo,