Stem cell separation technologies

Stem cell therapy and translational stem cell research require large-scale supply of stem cells at high purity and viability, thus leading to the development of stem cell separation technologies. This review covers key technologies being applied to stem cell separation, and also highlights exciting new approaches in this field. First, we will cover conventional separation methods that are commercially available and have been widely adapted. These methods include fluorescence-activated cell sorting (FACS), magnet-activated cell sorting (MACS), preplating, conditioned expansion media, density gradient centrifugation, field flow fractionation (FFF), and dielectrophoresis (DEP). Next, we will introduce emerging novel methods that are currently under development. These methods include improved aqueous two-phase system, systematic evolution of ligands by exponential enrichment (SELEX), and various types of microfluidic platforms. Finally, we will discuss the challenges and directions toward future breakthroughs for stem cell isolation. Advancing stem cell separation techniques will be essential for clinical and research applications of stem cells.

► Stem cell isolation methods that provide functional, viable stem cells are needed. ► Traditional stem cell separation platforms and their limitations. ► Emerging stem cell separation methods improve and complement traditional protocols. ► Several challenges still remain in stem cell isolation.