Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1760514 | Ultrasound in Medicine & Biology | 2013 | 12 Pages |
Abstract
With the emergence of the mouse as the predominant model system for studying mammalian brain development, in utero imaging methods are urgently required to analyze the dynamics of brain growth and patterning in mouse embryos. To address this need, we combined synthetic focusing with a high-frequency (38-MHz) annular-array ultrasound imaging system for extended depth-of-field, coded excitation for improved penetration and respiratory-gated transmit/receive. This combination allowed non-invasive in utero acquisition of motion-free 3-D data from individual embryos in approximately 2Â min, and data from four or more embryos in a pregnant mouse in less than 30Â min. Data were acquired from 148 embryos spanning 5Â d of early to mid-gestational stages of brain development. The results indicated that brain anatomy and cerebral vasculature can be imaged with this system and that quantitative analyses of segmented cerebral ventricles can be used to characterize volumetric changes associated with mouse brain development.
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Authors
Orlando Aristizábal, Jonathan Mamou, Jeffrey A. Ketterling, Daniel H. Turnbull,