Article ID Journal Published Year Pages File Type
1764824 Advances in Space Research 2013 11 Pages PDF
Abstract

We have explored the role of Ca2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca2+]i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca2+]i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca2+]i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca2+ influx through stretch-activated channels, then results in microfilament disruption.

► Simulated weightlessness disrupts microfilaments in osteoblasts. ► Simulated weightlessness inhibits the osteoblastic proliferation. ► Simulated weightlessness inhibits the migration of osteoblasts. ► Simulated weightlessness induces Ca2+ influx through stretch-activated channels. ► Ca2+ influx causes microfilament disruption induced by simulated weightlessness.

Related Topics
Physical Sciences and Engineering Earth and Planetary Sciences Space and Planetary Science
Authors
, , , , , , , ,