Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1767600 | Advances in Space Research | 2007 | 4 Pages |
Abstract
The objective of this study was to measure differences in the chromosomal rearrangements found in the progeny of cells exposed to the same dose of X-rays or energetic iron ions, with emphasis on intra-chromosomal exchanges. We hybridized metaphase cells with human DNA probes specific for the p and q arms of the chromosome 1. The arm-specific probes allow a fast and reliable detection of both symmetrical and asymmetrical inter-chromosomal exchanges and inter-arm intra-changes (pericentric inversions) in the painted chromosome pair. We used this method to score aberrations in human peripheral blood lymphocytes exposed to 1Â Gy of either 250Â kVp X-rays or 1Â GeV/n Fe-ions and harvested following 120Â h in culture, including 2Â h in colcemid for metaphase-block. Although iron ions are much more effective than X-rays in the induction of chromosomal aberrations formed during the first post-exposure cell cycle, we found that the effectiveness drops when daughter cells are scored at a late harvest time. In fact, no significant difference in the yield of simple interchanges or intra-changes was found for the two radiation qualities. On the other hand, complex-type exchanges, including rearrangements involving both intra- and inter-chromosomal exchanges, were much more frequent in the progeny of the population exposed to Fe-nuclei than to photons.
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Authors
L. Manti, A. Bertucci, G. Gialanella, G. Grossi, D. Pignalosa, M. Pugliese, P. Scampoli, M. Durante,