| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 176788 | Dyes and Pigments | 2012 | 7 Pages |
We report on the colorimetric and fluorimetric responses of the cationic water-soluble polythiophene derivative poly[3-(2-(N-(N′-methylimidazole))ethoxy)-4-methylthiophene] in presence of different phosphates with a focus on ATP. UV/Vis absorbance and fluorescence measurements show that ATP concentrations can be determined in aqueous solutions with a sufficient selectivity to other phosphates. Measurements of circular dichroism reveal that the polymer forms a random coil structure that exhibits no signal in the CD spectrum. Upon addition of ATP the conformation changes to a helix, yielding in strong CD peaks. These experimental data suggest that this polymer is an attractive, simple and versatile colorimetric and fluorimetric probe for nucleoside triphosphates such as ATP.
► Methylimidazolium-derived polythiophene as molecular probe for ATP recognition. ► Specific colorimetric, fluorimetric and CD response. ► Fluorescence quenching by aggregation of polymers in presence of triphosphates. ► Additional quenching effect by guanosine.
