Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography

•Lysozyme crystals were observed by X-ray digital topography using CCD camera.•Local rocking curves were observed by sequential digital topographic images.•Single, double, and multiple peaks of local rocking curves were observed.•Mapping of a full width of 10% maximum of local rocking curves was obtained.•Distribution of distortion of lysozyme crystals was investigated.

The crystal distortion of monoclinic hen egg-white lysozyme (HEWL) crystals was observed by the X-ray digital topography. The local rocking curves were obtained by the series of the topographic images. The single, double, triple, and multiple peaks of the local rocking curves were observed. The mapping of the full width of the one tenth maximum (FW10M) of the local rocking curves was obtained. It was found that the magnitude of FW10M depended on the distance of the peak splitting. The distribution of the distortion in the monoclinic HEWL crystals was investigated by the analysis of the local rocking curves.