| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1791210 | Journal of Crystal Growth | 2013 | 4 Pages |
Crystallization of membrane proteins in magnetic fields is thought to reveal the magnetic orientations of crystals, and is expected to enhance crystal quality for X-ray crystallographic analysis. The light-harvesting complex 2 (LH2) from a photosynthetic bacterium, Thermochromatium tepidum was crystallized in steep-gradient magnetic fields. The rod-shaped crystals of LH2 grown in the magnetic fields were oriented parallel to the magnetic field direction. An X-ray diffraction experiment indicated that the overall R value and crystal mosaicity are improved for the magnetically oriented crystal, and the helix bundles of LH2 were located parallel to the magnetic field direction in the crystal packing.
► Magnetic orientation of the crystals of a membrane protein was observed. ► Overall R value and crystal mosaicity of the oriented crystals were improved. ► Helix axis of the molecule was parallel to the magnetic field direction.
