Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1803904 | Journal of Magnetism and Magnetic Materials | 2008 | 11 Pages |
Abstract
Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocytes, chondrocytes, osteoblasts, melanocytes, macrophages, lung fibroblasts, and embryonic fibroblasts. These cells differed in several properties, and hence were a good representative sample of cells that would be targeted for expression and delivery of therapeutic genes in vivo. The efficiency of gene delivery in all these cells was enhanced using polyethylenimine-coated polyMAG magnetic nanoparticles, and the rates (17-84.2%) surpassed those previously achieved using other methods, especially in cells that are difficult to transfect. The application of permanent and pulsating magnetic fields significantly enhanced the transfection efficiencies in synoviocytes, chondrocytes, osteoblasts, melanocytes and lung fibroblasts, within 5Â min of exposure to these magnetic fields. This is an added advantage for future in vivo applications, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Condensed Matter Physics
Authors
Sarah W. Kamau Chapman, Paul O. Hassa, Sabine Koch-Schneidemann, Brigitte von Rechenberg, Margarethe Hofmann-Amtenbrink, Benedikt Steitz, Alke Petri-Fink, Heinrich Hofmann, Michael O. Hottiger,