Article ID Journal Published Year Pages File Type
1804223 Journal of Magnetism and Magnetic Materials 2007 4 Pages PDF
Abstract

Using GoldMag (Fe3O4/Au)(Fe3O4/Au) nanoparticles as a carrier, a biotin–avidin amplified ELISA was developed to detect hepatitis B surface antigen (HBsAg). A specific antibody was labeled with biotin and then used to detect the antigen with an antibody coated on GoldMag nanoparticles by a sandwich ELISA assay. The results showed that 5 mol of biotin were surface bound per mole of antibody. The biotin–avidin amplified ELISA assay has a higher sensitivity than that of the direct ELISA assay. There is 5-fold difference between HBsAg positive and negative serum even at dilution of 1:10000, and the relative standard deviation of the parallel positive serum at dilution of 1:4000 is 5.98% (n=11)(n=11).

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Physical Sciences and Engineering Physics and Astronomy Condensed Matter Physics
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