Article ID Journal Published Year Pages File Type
1806114 Magnetic Resonance Imaging 2016 7 Pages PDF
Abstract

In the present work, we reported a new nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) signal at around − 1.6 ppm (NOE(− 1.6)) in rat brain and investigated its application in the detection of acute ischemic stroke in rodent model. Using continuous wave (CW) MT sequence, the NOE(− 1.6) is reliably detected in rat brain. The amplitude of this new NOE signal in rat brain was quantified using a 5-pool Lorentzian Z-spectral fitting method. Amplitudes of amide, amine, NOE at − 3.5 ppm (NOE(− 3.5)), as well as NOE(− 1.6) were mapped using this fitting method in rat brain. Several other conventional imaging parameters (R1, R2, apparent diffusion coefficient (ADC), and semi-solid pool size ratio (PSR)) were also measured. Our results show that NOE(− 1.6), R1, R2, ADC, and APT signals from stroke lesion have significant changes at 0.5–1 h after stroke. Compared with several other imaging parameters, NOE(− 1.6) shows the strongest contrast differences between stroke and contralateral normal tissues and stays consistent over time until 2 h after onset of stroke. Our results demonstrate that this new NOE(− 1.6) signal in rat brain is a new potential contrast for assessment of acute stroke in vivo and might provide broad applications in the detection of other abnormal tissues.

Related Topics
Physical Sciences and Engineering Physics and Astronomy Condensed Matter Physics
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