Electrochemical determination of guanosine 5′-monophosphate using the electropolymerized film of non-peripheral amine substituted nickel(II) phthalocyanine modified electrode

This paper describes that the glassy carbon electrode (GCE) modified with a polymer film of non-peripheral amine substituted nickel(II) phthalocyanine (p-4α-NiIITAPc) can be used for the selective and stable determination of guanosine 5′-monophosphate (GMP) in phosphate buffer (pH 4) solution. Bare GCE oxidizes GMP at 1.26 V whereas p-4α-NiIITAPc modified GCE oxidizes it at 1.19 V with enhanced oxidation current. Further, the polymer modified electrode selectively determines GMP in the presence of 400-fold higher concentration of guanine. In differential pulse voltammetry measurements, the current response of GMP was linearly increased with the concentration range of 0.005–1 mM. The experimental detection limit was found to be 5 × 10−6 M.

► The p-4α-NiIITAPc film was used for the determination of GMP. ► Simultaneous determination of guanine and GMP was achieved. ► A detection limit of 5 × 10−6 M GMP was obtained.