Article ID Journal Published Year Pages File Type
1876385 Applied Radiation and Isotopes 2011 7 Pages PDF
Abstract

Various aspects of radiopeptide receptor-mediated cell internalisation and externalisation assays were assessed, including the integrity of externalised peptides and the effect of varying the pH and incubation time of the acid wash step (to remove surface receptor-bound ligand) on efficacy and cell viability. The observed intact proportion of externalised peptide was 5–10%, and acid wash buffers with pH 2.8 or below were found to be detrimental to cell viability and integrity, particularly following prolonged incubation times.

Related Topics
Physical Sciences and Engineering Physics and Astronomy Radiation
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