Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1876385 | Applied Radiation and Isotopes | 2011 | 7 Pages |
Abstract
Various aspects of radiopeptide receptor-mediated cell internalisation and externalisation assays were assessed, including the integrity of externalised peptides and the effect of varying the pH and incubation time of the acid wash step (to remove surface receptor-bound ligand) on efficacy and cell viability. The observed intact proportion of externalised peptide was 5–10%, and acid wash buffers with pH 2.8 or below were found to be detrimental to cell viability and integrity, particularly following prolonged incubation times.
Keywords
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Radiation
Authors
Syed Ali Raza Naqvi, Jane K. Sosabowski, Saeed Ahamad Nagra, Malik M. Ishfaq, Stephen J. Mather, Torkjel Matzow,