Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1877338 | Applied Radiation and Isotopes | 2006 | 6 Pages |
Abstract
This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity—reference activity). The affinities obtained for different ligand–receptor interactions agreed with affinities reported in the literature.
Related Topics
Physical Sciences and Engineering
Physics and Astronomy
Radiation
Authors
Henrik Björke, Karl Andersson,